Disposable culture systems are becoming increasingly prevalent compared to 10 years ago when it was used as a one-off production. Although, “classic” fermenters made from steel are still used everywhere, as they continue to equip their production facilities. Especially, due to their reliability and durability, alongside the main standard technological characteristics in design and processes.
Regarding the current state of the industry, many manufacturers of machinery and equipment, continue to provide consumers with a convenient service and product. As manufactures focus on the value of the product, place of production in terms of regulation standards, design and keeping silent about the “cost of ownership.” In order to reduce the cost of goods, the equipment is assembled with spare parts from 3rd party manufacturers. Especially, as this is does not catch the eye of the consumer, the equipment is assembled in large indivisible units.
The COVID-19 pandemic has affected many areas of life, including the development, market launch and production of life-saving pharmaceuticals. Demand for equipment and consumables for the production of monoclonal antibodies, viral vectors and other biopharmaceuticals has grown exponentially.
Membrane filtration is a pressure separation process that uses membranes to separate components in a liquid solution or suspension based on their size and differences in charge. Ultrafiltration is a type of membrane filtration in which hydrostatic pressure forces liquid against a semi-permeable membrane. Solvent and low molecular weight solutes pass through the membrane and are called permeate, while suspended solids and high molecular weight solutes are retained (retentate).
The stirrer is one of the most important components of fermenters and bioreactors. The optimal design of the stirrer (the number of blades and their position), as well as the speed of its rotation, ensure the best mass transfer during cell cultivation.
Fermentation is understood as the whole set of sequential operations from the introduction of seed material into a pre-prepared and heated medium to the required temperature and to the completion of the process of cell growth or biosynthesis of the target product. At the end of fermentation, a complex mixture is formed, consisting of producer…
The recent revival of interest in microbial fermentation for the production of biologics can be attributed to several key factors.
Fundamentals of Fermentation Fermentation encompasses a series of sequential operations, beginning with the introduction of seed material into a pre-prepared and thermally regulated medium, followed by the progression of cell growth or biosynthesis of the desired product. Upon completion of the fermentation process, a complex mixture is formed, comprising producer cells, residual nutrients, and biosynthetic…
From a technological perspective, fermentation processes can be classified into the following microbiological methods: aerobic and anaerobic cultivation; solid-phase, surface, and deep cultivation; as well as batch and continuous cultivation. This article will cover surface and deep cultivation, as well as batch and continuous cultivation. Surface Fermentation on Liquid Substrates. Surface fermentation is conducted in…
In industrial biotechnology, mechanically agitated fermenters serve as the foundation for large-scale microbial cultivation. Their design significantly influences process efficiency, product yield, and overall operational stability. This article explores the critical aspects of fermenter design, functionality, and future advancements.
The design of bioreactors is primarily determined by the method of energy supply and aeration of the medium. Based on these criteria, bioreactors can be categorized into the following types:
The development of antibody drugs relies on a well-structured Quality by Design (QbD) approach for upstream production. By leveraging the advantages of Design of Experiments (DoE) and high-throughput parallel bioreactor technology, researchers can efficiently complete laboratory-scale R&D processes. A critical step in this process is the early identification of the relationship between critical quality attributes…
